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KMID : 0363320170380020353
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Journal of Korean Oriental Internal Medicine
2017 Volume.38 No. 2 p.353 ~ p.366
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Effects of Gwaruhaengryeon-hwan on COPD and Particulate Matter Induced Lung Injury on a Mouse Model
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Lee Chul-Wha
Yang Won-Kyung
Lyu Yee-Ran
Kim Seung-Hyung
Park Yang-Chun
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Abstract
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Objective: This study aimed to use a mouse model to evaluate the effects of Gwaruhaengryeon-hwan (GHH) on chronic obstructive pulmonary disease (COPD) and particulate matter induced lung injury.
Materials and Methods: The study was carried out in two ways (in vitro, in vivo). In vitro RAW 264.7 cells (mouse macrophage) were used and analyzed by flow cytometry, ELISA. In vivo lipopolysaccharide (LPS) and cigarette smoke solution (CSS), or coal, fly ash, diesel exhaust particle (CFD) challenged mice were used and its BALF was analyzed by ELISA, lung tissue by real-time PCR.
Results: In vitro, GHH maintained an 80-100% rate of viability. So cytotoxicity was not shown. In the ELISA analysis with RAW 264.7 cells, GHH significantly decreased NO over 30 μg/ml. In the ELISA analysis, GHH significantly decreased TNF-α, IL-6 over 300 μg/ml. In the COPD model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increasing of neutrophils, TNF-α, IL-17A, MIP2, CXCL-1 in BALF, TNF-α, IL-1β mRNA expression in lung tissue and histological lung injury. In the CFD induced lung injury model, the GHH 200 mg/kg dosage group, the application of GHH significantly decreased the increase of neutrophils, TNF-α, IL-17A, MIP2, CXCL-1 in BALF, MUC5AC, TGF-β mRNA expression in lung tissue and histological lung injury.
Conclusion: This study suggests the usability of GHH for COPD patients by controlling lung tissue injury.
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KEYWORD
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Gwaruhaengryeon-hwan, chronic obstructive pulmonary disease, cigarette smoke solution, particulate matter
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